Research associated with the Bolus Herbarium covers a wide spectrum focused on aspects of the Cape Flora such as endemism, biogeography and systematics and evolution.
Systematic and evolutionary work is current on the following groups:
- Aizoaceae, C. Klak
- Apocyncaceae, Crassulaceae, Euphorbiaceae, P.V. Bruyns
- Bryophytes, T. Hedderson
- Cyperaceae, M. Muasya
- Psoralea, Otholobium & Aspalathus, C.H.Stirton & M. Muasya
- Asteraceae, Cyperaceae, Poaceae, T. Verboom