UCT MCB Triple Quad GC-MS
GC is the preferred technique for the separation of low molecular weight metabolites which are either volatile or can be converted into volatile and thermally stable compounds through chemical derivatization before analysis. This includes primary metabolites, such as amino acids, amines, sugars, organic acids and fatty acids. GC is not the preferred method for secondary metabolites.
The coupling of GC to electron impact ionization (EI) MS is a hybrid technique in analytical chemistry and is considered to be one of the most robust and highly sensitive instrument platforms for metabolite analysis.
MCB’s Triple Quad GC MS is the ideal instrument to be used for compound target analysis.
MCB Instruments
GC: Agilent 7890 (split inlet)
MS/MS: Agilent 7000C (electron ionization EI)
Carrier gas: Helium
Collison gas: Nitrogen
Columns
Agilent DB-5ms+DG, 30 meters, 0.250 mm (I.D.), 0.25 µm (film) up to 325°C (5% Phenyl-Arylene-95% methylpolysiloxane) + back flush column for non-polar & mixed-polarity compounds
| Cost of GC use | R360.00/hr |
| Setup of GC runs by MCB scientific officer | R360.00/hr |
| Training / assistance / method development / derivatization / report | R 360.00/hr |
| Consumables | |
| Vials | R10.00/vial |
| Vial caps | R5.00/cap |
| Vial inserts (0.25 mL-tapered bottom) | R5.00/insert |
| Vial inserts (0.25 mL-with polymer feet) | R30.00/insert |
| Nylon hydrophilic syringe filter (0.22 µm 13 mm) | R6.00/filter |
| Syringe (1 mL) | R5.00/syringe |
| 1.5 ml screw cap microfuge tubes | R8.00/cap+tube |
| Chemicals | |
| C8-C40 Alkanes | R50.00/10 µl |
| MSTFA | R50.00/100 µl |
| BF3 in methanol | R10.00/100 µl |
| BSTFA | R50.00/100 µl |
| External Academics | Total cost + 15% Vat |
| Industry | (Total cost + 30%) +15% Vat |
Contact
For enquiries, please contant Pei-Yin Liebrich (pei-yin.liebrich@uct.ac.za).